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| Registros recuperados: 14 | |
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| TEDESCO,MARÍLIA; KUHN,ANDRIELLE W.; FRESCURA,VIVIANE DAL-SOUTO; BOLIGON,ALINE A.; ATHAYDE,MARGARETH L.; TEDESCO,SOLANGE B.; SILVA,ANTONIO C.F. DA. |
| ABSTRACT The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy’s solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicinal plants; Mitotic index; Allium cepa; Sabugueiro; High-performance liquid chromatography. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502141 |
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| WANDSCHEER,ALANA C.D.; MARCHESAN,ENIO; TEDESCO,SOLANGE B.; FRESCURA,VIVIANE DAL-SOUTO; SOARES,CAMILLE F.; LONDERO,GUILHERME P.; TELÓ,GUSTAVO M.; HANSEL,DÂMARIS S.S.. |
| ABSTRACT Tricyclazole is currently one of the fungicides recommended for the treatment of diseases in irrigated rice. However, there is relatively little information on its cytotoxic and genotoxic potential. The objective of this study was to evaluate the cytotoxicity and genotoxicity of rice crop water after apllication of the tricyclazole fungicide through the Allium cepa L. test. The rice crop water samplings were collected before and 1, 15 and 30 days after application of the fungicide in rice plant shoots. The Allium cepa roots were placed in contact with the rice crop water to check for possible chromosomal abnormalities and mitotic index of the bioindicators meristematic cells. The data obtained by the Allium cepa test indicates that the application... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioindicator; Chromosomal abnormalities; Mitotic index; Pesticide; Irrigated rice. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301251 |
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| SILVA,M.S.A.; YAMASHITA,O.M.; ROSSI,A.A.B.; CONCENÇO,G.; CARVALHO,M.A.C.; FELITO,R.A.. |
| ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abnormalities; Bioassays; Cell division; Phasey bean; Mitotic index. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244 |
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| Lima,Eleonidas Moura; Rissino,Jorge Dores; Guimarães,Adriana Costa; Overal,David James; Khayat,André Salim; Souza,Patrícia Carvalho de; Christofolini,Denise Maria; Smith,Marília de Arruda Cardoso; Burbano,Rommel Rodríguez. |
| The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hep-2 cell line; Drifter technique; Mitotic index. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500005 |
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| Erolu,Erhan H; Hamzaolu,Ergin; Aksoy,Ahmet; Budak,Ümit; Özkul,Yusuf. |
| Helichrysum sanguineum, Helichrysum pamphylicum, Helichrysum orientale, Helichrysum noeanum (Asteraceae) are medicinal plants. For centuries, they have been used as tea in Turkey because of their medicinal properties. So far no scientifc evidence has been found in a literature survey regarding the genotoxic effects of these plants. This work evaluated the genotoxic effects on human lymphocyte cultures induced by methanol extracts of these plants, assayed in different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL). According to the results, Helichrysum noeanum, Helichrysum pamphylicum and Helichrysum sanguineum induced the formation of micronuclei and decreased the mitotic and replication indexes. Helichrysum orientale did not affect these parameters,... |
| Tipo: Journal article |
Palavras-chave: Genotoxic; Helichrysum; Micronucleus; Mitotic index; Replication index. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200005 |
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| Luber,Jaquelini; Palmieri,Marcel J.; Botelho,Carolina M.; Rinaldo,Daniel; Andrade-Vieira,Larissa F.. |
| Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa ("purple") - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citogenetoxicity; Root growth; Mitotic index; Seed germination; IC50. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903 |
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| Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos. |
| Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
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| Ananthi,Rajagopal; Chandra,Nallathambi; Santhiya,Sathiyavedu T.. |
| The aqueous extract of Hemidesmus indicus roots was investigated for its in vivo antigenotoxic effect against cisplatin-induced cytogenetic damage. Swiss albino mice were administered with various doses of the extract either singly (50, 100 and 200 mg/kg body weight) or as split doses (10, 20 and 40 mg/kg bw/day) for five consecutive days by oral gavage. As endpoints, chromosome aberrations, micronuclei in polychromatic erythrocytes, mitotic index and PCE/NCE ratio were estimated. The extract protected the bone marrow cells from cisplatin-induced genotoxicity in an inverse dose-dependent manner. However, the extract was cytotoxic at all doses. But, under split dose regime it conferred a higher level of genoprotection and was not cytotoxic at the lower two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genoprotective; Cytotoxic; Chromosomal aberrations; Micronucleus; Mitotic index. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100030 |
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| Eroğlu,Halil Erhan. |
| In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considerable anticlastogenic and antigenotoxic effects as observed in vitro in human lymphocytes. Thus, it could be concluded that tea polyphenols protected the normal cells from genotoxic or carcinogenic agents, which indicated the therapeutic and antioxidative role of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tea; Catechins; Micronucleus; Mitotic index; Replication index. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600011 |
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| Registros recuperados: 14 | |
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